Loop-mediated Isothermal Amplification (LAMP) uses 4–6 primers recognizing 6–8 distinct regions of target DNA for highly specific amplification. LAMP reactions occur under isothermal conditions without special thermal cyclers.
Key features: Four core primers + two loop primers for accelerated reaction. DNA products are >20 kb formed from numerous repeats of the 200–350 bp target sequence.
Retrieve sequence from NCBI by accession
— chromosomes (NC_), mRNA (NM_), RefSeq contigs
Upload local FASTA file
— plain FASTA, multi-FASTA, or sequences
Primer Design Options
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Need details? Sequence markup, parameters, output columns and the suggested workflow are documented in Help: LAMP tool · Help: Troubleshooting