Virtual (in silico) or electronic PCR (ePCR) for primers/probes, miRNA or gRNA binding site prediction. Eliminates "off-target" effects against whole genomes, predicts PCR products and searches for potential mismatches.
Alternative: For unlimited genome sizes, use the command-line in silico PCR Java application.
Upload or paste sequence(s) in FASTA format:
Target sequence(s)
Primers list
Results
PCR amplicons
Amplicon Detection Parameters
Degenerate codes:
LNA:
B=CGT, D=AGT, H=ACT, K=GT, M=AC, N=ACGT, R=AG, S=GC, V=ACG, W=AT, Y=CT, U=Uracil, I=Inosine.
LNA:
E=dA, F=dC, J=dG, L=dT.
Output: Export: Select all (Ctrl+A), copy (Ctrl+C), paste (Ctrl+V)
Quick Help for In Silico PCR (ePCR)
- Provide target sequence(s) in FASTA or retrieve by NCBI accession; paste primer list in the second tab.
- Use degenerate/IUPAC primers if needed; check mismatches/off-targets in the results tabs.