FastPCR

Documentation and reference pages for primer design, assay planning, and sequence analysis workflows.

Use this hub to access quick command help, practical examples (LAMP, LATE-PCR, SSR, Ta prediction), citations, and module-specific pages.

Product overview Open documentation
Tip: bookmark this page as your “landing” point when switching between modules.
FastPCR

Help

Quick PCR command reference and design options.

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Examples

Practical design workflows and parameter sets.

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PCR optimization

Template, primer, buffer and cycling guidelines.

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Citations

How to cite FastPCR in publications.

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License

Academic, government, and commercial licensing options.

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Modules

Open module pages →
Module 1

Inputs to FastPCR

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Module 2

Sequence entry and file formats

Sequence input and the file formats FastPCR reads.

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Module 3

Standard Menu Toolbar

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Module 4

Primers analysis and compatibility

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Module 5

PCR primers design generalities

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Module 6

PCR primers and probes design command lines

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Module 7

Melting temperature calculation

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Module 8

Calculation of optimal annealing temperature

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Module 9

Prepare for PCR analysis

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Module 10

Program output

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Module 11

The secondary non-specific binding test; alternative amplification

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Module 12

Multiplex primer design

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Module 13

In silico or virtual PCR or primers (probes) searching

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Module 14

Group-specific (family-specific primer set) and Unique PCR primers design

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Module 15

Probes design

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Module 16

PCR amplification protocol

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Module 17

Bioinformatics Tools

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Module 18

Database

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Module 19

Alignment Search

Sequence alignment and similarity search.

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Module 20

The Restriction Endonuclease Analysis

Restriction site search and digest prediction.

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Module 21

Citing FastPCR and references

How to cite FastPCR, plus the literature the algorithms are based on.

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Module 22

Need help?

Where to get clarifications, examples and support.

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Navigation note
Module pages share a common layout and can be linked from internal protocols, papers, and lab SOPs.