FastPCR
An integrated platform for PCR primer and probe design, in silico PCR, oligonucleotide analysis, alignment, and repeat detection.
FastPCR Documentation
Complete manuals, module descriptions, and usage examples are available in the documentation section.
Key capabilities
FastPCR consolidates primer design, assay planning, and sequence analysis in a single workflow, supporting standard PCR as well as specialized genotyping and isothermal amplification assays.
Primer and assay design
- PCR primer design for standard, long-distance, inverse, and real-time PCR (TaqMan, LUX, Molecular Beacon), multiplex PCR, and Xtreme Chain Reaction (XCR®).
- Single-primer PCR design from closely located inverted repeats; STR locus detection with direct primer design; bisulfite PCR primer design; degenerate PCR from amino acid sequences.
- Loop-mediated isothermal amplification (LAMP).
- Allele-specific assays: KASP™ (how it works) and PACE™ (assay overview).
- castPCR™ / mutation detection workflows (Thermo Fisher), and SNaPshot® multiplex SNP genotyping (overview).
- MLPA® probe design (MRC Holland) and Invader® assay references (paper).
Assembly, QC, and analysis
- Fragment assembly using Gibson Assembly and polymerase cycling assembly (PCA).
- Design of overlapping/non-overlapping amplicons tiling regions of interest for targeted NGS workflows.
- In silico PCR and oligo search against genomes/chromosomes with mismatch discovery and Tm/annealing support.
- Comprehensive oligo QC: Tm for standard/degenerate oligos, primer efficiency, linguistic complexity, dilution/resuspension calculators.
- Secondary structure analysis including hairpins, self-/cross-dimers, G-quadruplexes and wobble pairs.
- Sequence analysis: skew metrics, GC/GA content, random sequence generation (Tm/GC constrained), restriction enzyme analysis (REBASE), alignments/contigs/consensus, k-mer distance, and tandem repeat detection (tandem repeats).
Custom features
If you need a feature that is not available in the current release, please contact the team to discuss customization.
System requirements
FastPCR is available for Microsoft Windows and works best on systems meeting the minimum specifications below.
- Microsoft Windows
- 2 GB RAM
- 1280×800 minimum screen resolution
If your system does not meet these requirements, some features may be limited.
Download and installation
- Current version
- 7.0.03
- License type
- Trial
Installation steps
- Download and save FastPCR.msi on your computer.
- Run FastPCR.msi to start the installation wizard.
- Follow the wizard steps. Administrator rights may be required.
The trial is activated for 7 days. After the evaluation period, the program can be removed via Add/Remove Programs in the Windows Control Panel.
Training and certification
Training solutions cover FastPCR and WebTools. Certification options validate skills in FastPCR and PCR application development.