Test for Primer:  
14 5'-cgggcatccatccaatgcattctt 
 
 
          <-ttcttacgtaacctacctacgggc-5 
               ||||||||    
5-cgggcatccatccaatgcattctt-> 
Tm=10.2°C 
 
                 Test for Primer:  
22 5'-tggttgttgtggtagacgtctcgt 
 
 
          <-tgctctgcagatggtgttgttggt-5 
               ||||||||    
5-tggttgttgtggtagacgtctcgt-> 
Tm=19.3°C 
 
                 Test for Primer:  
23 5'-cgagacgtctaccacaacaaccaa 
 
 
<-aaccaacaacaccatctgcagagc-5 
                ||||||||   
            5-cgagacgtctaccacaacaaccaa-> 
Tm=24.7°C 
 
                 Test for Primer:  
27 5'-cggaaagactaggcctactaaaggca 
 
 
<-acggaaatcatccggatcagaaaggc-5 
   |     | |||||||| |     |  
5-cggaaagactaggcctactaaaggca-> 
Tm=4.0°C 
 
                 Test for Primer:  
36 5'-ggttgttgtggtagacgtctcgtt 
 
 
        <-ttgctctgcagatggtgttgttgg-5 
              ||||||||     
5-ggttgttgtggtagacgtctcgtt-> 
Tm=19.3°C 
 
                 Test for Primer:  
57 5'-gccggaaagactaggcctactaaa 
 
 
      <-aaatcatccggatcagaaaggccg-5 
           | |||||||| |    
5-gccggaaagactaggcctactaaa-> 
Tm=4.0°C 
 
                 Test for Primer:  
58 5'-tgccggaaagactaggcctactaaag 
 
 
      <-gaaatcatccggatcagaaaggccgt-5 
            | |||||||| |     
5-tgccggaaagactaggcctactaaag-> 
Tm=4.0°C 
 
                 Test for Primer:  
118 5'-aaccgatcgatctagccatgagca 
 
 
<-acgagtaccgatctagctagccaa-5 
              ||||||||     
        5-aaccgatcgatctagccatgagca-> 
Tm=19.5°C 
 
                 Test for Primer:  
145 5'-tctgcagattcgcaatcagagcct 
 
 
<-tccgagactaacgcttagacgtct-5 
                  |||||||| 
                5-tctgcagattcgcaatcagagcct-> 
Tm=27.8°C 
 
                 Test for Primer:  
167 5'-tgcattgcatacggctccacaatg 
 
 
  <-gtaacacctcggcatacgttacgt-5 
    |||||   |    |   ||||| 
5-tgcattgcatacggctccacaatg-> 
Tm=6.9°C 
 
                 Test for Primer:  
205 5'-gatgcatcgaatggacgcgaagtt 
 
 
<-ttgaagcgcaggtaagctacgtag-5 
                  |||||||| 
                5-gatgcatcgaatggacgcgaagtt-> 
Tm=24.8°C 
 
                 Test for Primer:  
220 5'-accaccggtgaggtatacacgaaa 
 
 
<-aaagcacatatggagtggccacca-5 
                ||||||||   
            5-accaccggtgaggtatacacgaaa-> 
Tm=31.1°C 
 
                 Test for Primer:  
221 5'-aaccaccggtgaggtatacacgaa 
 
 
<-aagcacatatggagtggccaccaa-5 
               ||||||||    
          5-aaccaccggtgaggtatacacgaa-> 
Tm=31.1°C 
 
                 Test for Primer:  
222 5'-aaaccaccggtgaggtatacacga 
 
 
<-agcacatatggagtggccaccaaa-5 
          |   ||||||||   | 
        5-aaaccaccggtgaggtatacacga-> 
Tm=34.7°C 
 
                 Test for Primer:  
227 5'-tgtctgtacaaacgagaacggcct 
 
 
<-tccggcaagagcaaacatgtctgt-5 
             || |||||| ||  
          5-tgtctgtacaaacgagaacggcct-> 
Tm=9.8°C 
 
                 Test for Primer:  
242 5'-atatcaacagctgtggcaatcggc 
 
 
<-cggctaacggtgtcgacaactata-5 
      ||  | |||||||| |  || 
    5-atatcaacagctgtggcaatcggc-> 
Tm=22.9°C 
 
                 Test for Primer:  
247 5'-ccattgcaattgccggaaagactagg 
 
 
<-ggatcagaaaggccgttaacgttacc-5 
               ||||||||      
        5-ccattgcaattgccggaaagactagg-> 
Tm=23.1°C 
 
 
<-ggatcagaaaggccgttaacgttacc-5 
                | |||||||| | 
              5-ccattgcaattgccggaaagactagg-> 
Tm=9.5°C 
 
                 Test for Primer:  
292 5'-gcatatgcctgctttgaccgagat 
 
 
<-tagagccagtttcgtccgtatacg-5 
                  |||||||| 
                5-gcatatgcctgctttgaccgagat-> 
Tm=29.4°C 
 
                 Test for Primer:  
318 5'-acagaacgttccgctactgacact 
 
 
<-tcacagtcatcgccttgcaagaca-5 
             | |||||||| |  
          5-acagaacgttccgctactgacact-> 
Tm=5.1°C 
 
                 Test for Primer:  
345 5'-cccttgtgatggtttcgcgaagtt 
 
 
          <-ttgaagcgctttggtagtgttccc-5 
               ||||||||    
5-cccttgtgatggtttcgcgaagtt-> 
Tm=4.0°C 
 
                 Test for Primer:  
370 5'-tccattgtggagccgtatgcaatg 
 
 
  <-gtaacgtatgccgaggtgttacct-5 
    |||||   |    |   ||||| 
5-tccattgtggagccgtatgcaatg-> 
Tm=24.5°C