Web Tools for Advanced PCR Design, Genotyping, LAMP and Sequence Analysis

Filter by technique, method, or tool name.

Design & Assays

Standard, inverse, multiplex, TaqMan, MGB-probe, bisulfite PCR and RPA assay design.

Custom amplicon panel for NGS and TGS targeted sequencing across organisms.

AS-PCR / KASP™ / PACE™ / ASQ genotyping for SNPs, InDels and haplotypes.

Isothermal amplification using 4–6 primers recognizing 6–8 distinct target regions.

Seamless isothermal assembly of multiple overlapping DNA fragments in a single reaction.

Virtual PCR for primers, probes, miRNA or gRNA binding prediction and off-target detection.

Oligo Analysis

Single-oligo analysis: Tm, GC%, MW, extinction coefficient, OD, complexity, self-dimers, G-quadruplexes.

Batch analysis of multiple primers: Tm, GC%, complexity, MW, self-dimers and cross-dimers.

Fluorescein / biotin probe pairs for AAV titer by biolayer interferometry (BLI).

Repeats & Genome

De novo identification, masking and clustering of repeats at genomic scale. Detects SSRs and interspersed repeats.

Needleman-Wunsch, Smith-Waterman and progressive MSA - all in the browser.

Calculators

Calculate reagent volumes for PCR, qPCR and LAMP. Supports 10+ polymerase presets.

Mix two solutions - molar, %, pH or any unit. Provide 4 of 6 variables; the rest are computed.

All computations run locally in your browser. Your sequences are never sent to any server.