PrimerDigital


Standard Menu Toolbar

Find Window – use F3, is the quick way to find exact word in general editor.

fastpcr

Menu toolbar


  • fastpcr Clean text at current open TAB editor (Ctrl-D)
  • fastpcr Open file into general editor (Ctrl-O)
  • fastpcr Load file(s) from the folder Ctrl-G directly into memory without opening files into editor; small or large text file with DNA sequence(s) at FASTA format or TAB/Space-column formatted text files
  • fastpcr Direct analysis of all file(s) (Ctrl-J) from the folder without opening files into editor; small or large text file with DNA sequence(s) at FASTA format or TAB/Space-column formatted text files
  • fastpcr Save text as Rich Text (.RTF) or Excel (.XLS) formats from opened TAB.
  • fastpcr For starting any job, need press F5 or from tool bar press

Quick sequence transformation

Tool for converting sequences to IUB/IUPAC FASTA format (Ctrl-F1);
Tool bar for converting the sequences to FASTA format with saving original sequence (alignment) (Ctrl-F2);


FastPCR program includes tools for the manipulation of DNA or protein sequences:

fastpcr

  • fastpcr Reverse (e.g. acacacc become ccacaca) (Ctrl-F3)
  • fastpcr Complement (e.g. acacacc become tgtgtgg) (Ctrl-F4)
  • fastpcr Reverse-Complement transformation (e.g. acacacc become ggtgtgt) (Ctrl-F5)
  • fastpcr DNA-to-protein translation in all the 6 reading frames; orientation, complementation of protein sequence, and protein-to-DNA translation (Ctrl-F6)
  • fastpcr Tool for joining all sequences into a single sequence (Ctrl-F7)
  • fastpcr Export data from opened Tab into Excel sheet (Ctrl-F8)
  • Names to Number List (Ctrl-F9) – tool for converting sequences to FASTA format with changing sequence’s name to number. Default is 1, to specify initial number, type in additional editor this number
  • Tab-Table Format (Ctrl-F11) – tool for converting sequences to column format with Tab separator between names
  • Blank-Table Format (Ctrl-F12) – tool for converting sequences to column format with space separator between names

In case the @ character at the beginning of the sequence name's, the result - the sequence will be translated into complementary (for Ctrl-1 and Ctrl-2), or remains the original seqience (for other cases).

  • fastpcr Selecting and extracting fragments tool for linear sequence. Using additional editor, you can specify region(s) for extracting from sequence(s). To select fragments needs to select a beginning and an end that will be the beginning and end of the produced fragment.

Example, type on sequence description:
> 1 100 300 550
acgacgcagcagcacgacagt...

Output result extraction: the sub-fragments between 1 and 100 and second between 300 and 550.



Manual